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KMID : 0667719890260000417
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Report Natlonal Institute of Health
1989 Volume.26 No. 0 p.417 ~ p.426
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Studies on the Extraction Quantity of the Specific Components of Crude Drug Preparation Based on Prescription (¥²)
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ì°Ð¤áæ/Lee, K. S.
ð³ÐÑÖ§/ûóà¼øõ/ÚÓäÐФ/ÑÑæØâ×/Ùþܹóú/ÛÑÚ¸â×/Ze, K. R./Hong, S. P./Mun, B. C./Park, A. K./Kim, Y. S./Bae, M. S.
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Abstract
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Separation and quantitation of the aconitine alkaloids was done by HPLC. The method comprises extraction of aconite powder first with ammoniacal ether and then with methanol(X3 each), or extraction with water by reflux for 4hr, chromatography of the resultant extract over neutral alumina and elution with ethyl acetate-methanol (7:3) prior to HPLC.
The analytical conditions of H.P.L.C. were as follows.
¡¤column : ¥ì-Bondapak¢â C_(18) (300¡¿3.9mm I.D)
Guard column C_(18)(50¡¿4.0mm I.D)
¡¤Detector : UV (254nm)
¡¤mobile phase : THF ¡¤phosphate buffer(pH2.7) (11 : 89)
¡¤flow rate : 1.0ml/min
The method has been found to be suitable for routine analysis of aconite.
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